Association of phorbol ester-induced hyperphosphorylation and reversible regulation of transferrin membrane receptors in HL60 cells.
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Discussion
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Implication
In vitro study reveals reversible transferrin receptor internalization tied to hyperphosphorylation in HL60 cells, indicating an early signal for leukemic cell differentiation.
Key Points
- To determine the molecular mechanism controlling surface transferrin receptor expression during phorbol diester-induced differentiation of HL60 human leukemic cells.
- Treated human leukemic HL60 cells with tumor-promoting phorbol diesters to induce cell differentiation.
- Tracked the kinetics, reversibility, and internalization of cell-surface transferrin receptors.
- Assessed changes in the phosphorylation status of transferrin receptors in relation to receptor regulation.
- Phorbol diesters caused rapid and reversible internalization of membrane transferrin receptors in HL60 cells.
- Receptor internalization occurred in temporal association with reversible hyperphosphorylation of the transferrin receptor.
- Reversible surface receptor regulation acts as an early signaling event facilitating HL60 cell differentiation.
Cite This Study
May et al. (1984) studied this question.