What is the clinical evidence from this study?

Study design: Other. Intervention: Molecular cloning of macrophage NOS vs. Neuronal NOS. Primary outcome: Sequence identity and enzyme characteristics.

August 1, 1992Proceedings of the National Academy of SciencesOpen Access

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.

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Key result

Macrophage nitric oxide synthase was cloned, displaying 50% sequence identity to the neuronal enzyme and striking inducibility 2-6 hours after endotoxin treatment.

Population

RAW 264.7 macrophages, BALB/c mice, and human embryonic kidney 293 cells

Comparison

Molecular cloning of macrophage nitric oxide… vs Neuronal NOS; unstimulated cells/mice; antisense…

Design

Preclinical

Authors

CLCharles J. LowensteinVascular / Pulmonary Vascular CGC S GlattJohns Hopkins University DBDavid S. BredtUniversity of Maryland, Baltimore

Discussion

Member takes

Implication

Highlights distinct inducible macrophage NOS; leaves open its role in human inflammation and therapeutic targeting.

Structured PICO

Population

RAW 264.7 macrophages (monocyte-macrophage cell line from BALB/c mice), BALB/c mice, and human embryonic kidney 293 cells

Intervention

Molecular cloning of macrophage nitric oxide synthase (mac-NOS), transfection, and stimulation with lipopolysaccharide (LPS) and interferon gamma

Comparator

Neuronal NOS (n-NOS); unstimulated cells/mice; antisense mac-NOS or vector alone

Outcome

mac-NOS sequence identity, catalytic activity, and mRNA expression levels

The cloning of macrophage nitric oxide synthase demonstrates it is a distinct, highly inducible, calcium-independent isoform with 50% sequence identity to neuronal NOS.

Cite This Study

Lowenstein et al. (1992) studied this question. Molecular cloning of macrophage NOS vs. Neuronal NOS was evaluated on Sequence identity and enzyme characteristics. Macrophage nitric oxide synthase was cloned, displaying 50% sequence identity to the neuronal enzyme and striking inducibility 2-6 hours after endotoxin treatment.

synapsesocial.com/papers/6a0d43ea48a82a5ce309adba https://doi.org/10.1073/pnas.89.15.6711

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August 1, 1992Proceedings of the National Academy of SciencesOpen Access

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.

View Full Paper

Ask AI

Bookmark

Key result

Macrophage nitric oxide synthase was cloned, displaying 50% sequence identity to the neuronal enzyme and striking inducibility 2-6 hours after endotoxin treatment.

Population

RAW 264.7 macrophages, BALB/c mice, and human embryonic kidney 293 cells

Comparison

Molecular cloning of macrophage nitric oxide… vs Neuronal NOS; unstimulated cells/mice; antisense…

Design

Preclinical

Authors

CLCharles J. LowensteinVascular / Pulmonary Vascular CGC S GlattJohns Hopkins University DBDavid S. BredtUniversity of Maryland, Baltimore

Discussion

Member takes

Implication

Highlights distinct inducible macrophage NOS; leaves open its role in human inflammation and therapeutic targeting.

Structured PICO

Population

RAW 264.7 macrophages (monocyte-macrophage cell line from BALB/c mice), BALB/c mice, and human embryonic kidney 293 cells

Intervention

Molecular cloning of macrophage nitric oxide synthase (mac-NOS), transfection, and stimulation with lipopolysaccharide (LPS) and interferon gamma

Comparator

Neuronal NOS (n-NOS); unstimulated cells/mice; antisense mac-NOS or vector alone

Outcome

mac-NOS sequence identity, catalytic activity, and mRNA expression levels

The cloning of macrophage nitric oxide synthase demonstrates it is a distinct, highly inducible, calcium-independent isoform with 50% sequence identity to neuronal NOS.

Cite This Study

synapsesocial.com/papers/6a0d43ea48a82a5ce309adba https://doi.org/10.1073/pnas.89.15.6711

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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