Macrophage nitric oxide synthase was cloned, displaying 50% sequence identity to the neuronal enzyme and striking inducibility 2-6 hours after endotoxin treatment.
The cloning of macrophage nitric oxide synthase demonstrates it is a distinct, highly inducible, calcium-independent isoform with 50% sequence identity to neuronal NOS.
Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.
Lowenstein et al. (Sat,) reported a other. Molecular cloning of macrophage NOS vs. Neuronal NOS was evaluated on Sequence identity and enzyme characteristics. Macrophage nitric oxide synthase was cloned, displaying 50% sequence identity to the neuronal enzyme and striking inducibility 2-6 hours after endotoxin treatment.
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