Regulation of Simian Virus 40 Transcription: Sensitive Analysis of the RNA Species Present Early in Infections by Virus or Viral DNA
Population
Monkey cells infected with wild-type simian virus 40 (SV40), mutant tsA58 virus, and the corresponding DNAs
Comparison
Infection with wild-type SV40 virus or DNA, or… vs Comparison between wild-type and mutant tsA58…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Supports T antigen positive control of SV40 late transcription; leaves open extension beyond this animal model.
Structured PICO
P
Population
Monkey cells infected with wild-type simian virus 40 (SV40), mutant tsA58 virus, and the corresponding DNAs
I
Intervention
Infection with wild-type SV40 virus or DNA, or mutant tsA58 virus or DNA, evaluated at permissive (37 degrees C) and nonpermissive (41 degrees C) temperatures
C
Comparator
Comparison between wild-type and mutant tsA58 infections at different temperatures and time points
O
Outcome
Presence and amounts of specific early and late viral RNA species in cytoplasmic and nuclear fractionssurrogate
The findings suggest that SV40 large-T antigen plays an active positive role in late viral RNA synthesis, rather than late transcription being controlled by a negative repressor.
Cite This Study
Parker et al. (1979) studied this question.