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August 1, 1996Journal of Biological ChemistryOpen Access

Phosphorylation and Activation of Myosin by Rho-associated Kinase (Rho-kinase)

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Key result

Rho-kinase phosphorylates myosin light chain at Ser-19 and facilitates the actin activation of myosin ATPase, providing a mechanism for Rho-mediated smooth muscle contraction.

Population

In vitro biochemical models using recombinant myosin light chain (MLC)

Comparison

Rho-associated kinase (Rho-kinase) vs Mutant recombinant MLC

Design

Preclinical

Authors

MAMutsuki AmanoNagoya UniversityMIMasaaki ItoNihon UniversityKKKazushi KimuraHokkaido Bunkyo University

Discussion

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Implication

May guide Rho-kinase inhibitor use in vascular disorders; leaves open clinical translation from this animal mechanism.

Structured PICO

P
Population
In vitro biochemical models using recombinant myosin light chain (MLC)
I
Intervention
Rho-associated kinase (Rho-kinase)
C
Comparator
Mutant recombinant MLC (Ala substituted for Thr-18 and Ser-19)
O
Outcome
Phosphorylation of myosin light chain (MLC) and activation of myosin ATPasesurrogate

Rho-kinase directly phosphorylates myosin light chain at Ser-19 to activate myosin ATPase, providing a biochemical mechanism for Rho-mediated smooth muscle contraction and cell motility.

Cite This Study

Amano et al. (1996) studied this question. Rho-kinase was evaluated on Phosphorylation of myosin light chain (MLC) and activation of myosin ATPase. Rho-kinase phosphorylates myosin light chain at Ser-19 and facilitates the actin activation of myosin ATPase, providing a mechanism for Rho-mediated smooth muscle contraction.

synapsesocial.com/papers/6a0d440c88250cfcc2a4d8achttps://doi.org/10.1074/jbc.271.34.20246
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Microinjection of recombinant p21rho induces rapid changes in cell morphology.1990 · 719 citations
  2. 2Mutagenesis of the Phosphorylation Site (Serine 19) of Smooth Muscle Myosin Regulatory Light Chain and Its Effects on the Properties of Myosin1994 · 82 citations
  3. 3Phosphorylation of smooth muscle myosin at two distinct sites by myosin light chain kinase.1985 · 351 citations
  4. 4CONTROL OF NONMUSCLE MYOSINS BY PHOSPHORYLATION1992 · 341 citations
  5. 5Physiology of the Gastrointestinal Tract1988 · 969 citations