Removal of plasminogen from serum depressed the plating efficiency of SV-40-transformed cells in soft agar, eliminated their characteristic aggregation, and reduced migration.
Does plasminogen depletion affect the growth, aggregation, and migration of normal and SV-40-transformed cells?
Plasminogen plays a critical role in the characteristic morphological changes and migration of SV-40-transformed cells, but not in their exponential growth in liquid medium.
Fetal bovine and dog serum were selectively freed of plasminogen by affinity chromatography. The resulting serum as well as native and reconstituted serum (obtained by the addition of purified plasminogen to the plasminogen-depleted serum) were used to examine the role of plasminogen in (a) growth of normal and SV-40-transformed hamster embryo fibroblasts in liquid medium, (b) growth of SV-40-transformed hamster embryo fibroblasts in soft agar, (c) aggregation - a characteristic morphological change of SV-40-transformed hamster cells, and (d) migration of SV-40-transformed and control 3T3 cells from a monolayer into a "wound." The results demonstrated that exponential growth of both normal and transformed cells in liquid medium proceeded at the same rate in the presence or absence of plasminogen. In contrast, removal of plasminogen markedly depressed the plating efficiency of transformed cells in soft agar, eliminated their characteristic aggregation, and substantially reduced the extent of migration. The role of plasminogen and its activation in oncogenic transformation is discussed.
Ossowski et al. (Thu,) conducted a other in Oncogenic transformation (in vitro). Plasminogen-depleted serum vs. Native and reconstituted serum was evaluated on Cell growth, aggregation, and migration. Removal of plasminogen from serum depressed the plating efficiency of SV-40-transformed cells in soft agar, eliminated their characteristic aggregation, and reduced migration.