What question did this study set out to answer?

The research aims to develop a method for synthesizing a novel noncanonical amino acid, H-L-photo-lysine, and demonstrate its incorporation into proteins for studying interactions.

May 20, 2026ChemBioChemOpen Access

Facile Synthesis of H‐L‐Photo‐Lysine and Its Genetic Incorporation Into Proteins

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Authors

AHAbu HamzaVirginia Commonwealth University CSChiranjeev SharmaVirginia Commonwealth University YCYana CenVirginia Commonwealth University

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Implication

Randomized trial demonstrates enhanced protein labeling using H-L-photo-lysine for studying SIRT6 interactions.

Key Points

The research aims to develop a method for synthesizing a novel noncanonical amino acid, H-L-photo-lysine, and demonstrate its incorporation into proteins for studying interactions.
Synthesis of H-L-photo-lysine with improved yields and simplified purification
Genetic code expansion to incorporate photoK into a GFP variant
Photocrosslinking studies conducted with SIRT6K15photoK mutant in HEK293 and E. coli cell lysates
Successfully synthesized H-L-photo-lysine with improved yield and milder conditions
Confirmed UV-dependent crosslinking of SIRT6K15photoK with histone H3, indicating specific interactions
Pretreatment with a SIRT6-selective inhibitor significantly reduced crosslinking, confirming specificity of interactions

Cite This Study

Hamza et al. (2026) studied this question.

synapsesocial.com/papers/6a0d5013f03e14405aa9ba68 https://doi.org/10.1002/cbic.70388

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Facile Synthesis of H‐L‐Photo‐Lysine and Its Genetic Incorporation Into Proteins

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Implication

Key Points

Cite This Study

Facile Synthesis of H‐L‐Photo‐Lysine and Its Genetic Incorporation Into Proteins

Discussion

Member takes

Implication

Key Points

Cite This Study

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