Multispectral vs. RGB UAV Imagery for Detecting Mistletoe (Viscum album) in Scots Pine Forests: Identifying the Most Informative Vegetation Indices

The aim of this study was to examine the potential of multispectral imaging derived from unmanned aerial vehicles (UAVs) for detecting the spread of mistletoe (Viscum album ssp. austriacum L.) in Scots pine stands and to assess the information potential of selected vegetation indices in mistletoe detection. UAV campaigns were performed in the Niepołomice Primeval Forest (Niepołomice Forest District, Regional Directorate of the Polish State Forests National Holding, Kraków, Poland). A fixed-wing UAV Trinity F90+ (Quantum Systems GmbH) equipped with a five-band multispectral MicaSense RedEdge-M camera and an RGB Sony UMC-R10C camera was employed. The number of trees infected by mistletoe, as well as the quantity and area of mistletoe biogroups, were derived based on the classification of true multispectral orthophotos using a support vector machine (SVM) classifier. The spectral information potential assessment identified NIR (B5) as the most important single spectral source of information, while the greatest information potential among vegetation indices was found in NormG, CIG, and GRVI. The mistletoe classification of the 22.5-ha compartment revealed 1735 mistletoe biogroups covering a total area of 489 m2, with 58.6% of the 2917 detected tree crowns identified as infected (Kappa = 0.74). The results confirm that UAV-based multispectral data, particularly when combined with green-sensitive vegetation indices, enable effective differentiation of mistletoe from host tree crowns. The integration of the near-infrared (NIR) band further enhanced classification performance. This study evaluates UAV-based multispectral and RGB imagery for detecting common mistletoe (Viscum album ssp. austriacum) in Scots pine stands. The information potential of 22 vegetation indices was assessed to identify the most effective spectral features for mistletoe classification.