What question did this study set out to answer?

To characterize the mechanism of riboflavin uptake and investigate membrane-associated riboflavin-binding activity in Bacillus subtilis.

January 1, 1980Journal of Supramolecular Structure

Uptake and binding of riboflavin by membrane vesicles of bacillus subtilis

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To characterize the mechanism of riboflavin uptake and investigate membrane-associated riboflavin-binding activity in Bacillus subtilis.
Assayed riboflavin uptake, counterflow, and exchange mechanisms in isolated Bacillus subtilis membrane vesicles.
Solubilized the membrane-associated binding component using detergents and partially purified it to evaluate binding kinetics and competitor responses.
Riboflavin uptake and membrane-associated binding activity are coordinately repressed by riboflavin present in the growth medium.
Transport occurs via a carrier-mediated system demonstrating counterflow, exchange, and saturation kinetics that are inhibited by riboflavin analogues.
The partially purified binding component exhibits greater affinity for reduced riboflavin than for oxidized riboflavin.

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Implication

Biochemical study reveals carrier-mediated riboflavin uptake in Bacillus subtilis membrane vesicles, indicating redox-dependent regulation of bacterial vitamin transport.

Cite This Study

Cecchini et al. (1980) studied this question.

synapsesocial.com/papers/6a0d9cfa389a567298ba92ec https://doi.org/10.1002/jss.400130109

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Implication

Biochemical study reveals carrier-mediated riboflavin uptake in Bacillus subtilis membrane vesicles, indicating redox-dependent regulation of bacterial vitamin transport.

Uptake and binding of riboflavin by membrane vesicles of bacillus subtilis

Key Points

Discussion

Implication

Cite This Study

Uptake and binding of riboflavin by membrane vesicles of bacillus subtilis

Key Points

Discussion

Implication

Cite This Study

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