Key result
Heteromeric assembly of Shaker-like potassium channels is cotranslational, and N207 glycosylation of Kv1.1 occurs but is not required for subunit assembly, transport, or function.
Population
In vitro translations and transfected mouse L-cells
Comparison
Cotranslation of Kv1.1 and Kv1.4; mutation of… vs Sham-transfected cells; non-Shaker Kv2.1 channel
Design
Preclinical
Authors
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No immediate clinical implications; leaves open glycosylation requirements for native Kv1 channel function in mammals.
This basic science study demonstrates that heteromeric assembly of Shaker-like potassium channels is cotranslational and that N207 glycosylation is not required for channel function or transport.
Deal et al. (1994) studied this question. Kv1.1 and Kv1.4 channel expression / N207Q mutation vs. Sham-transfected cells / wild-type was evaluated on Subunit assembly and posttranslational processing. Heteromeric assembly of Shaker-like potassium channels is cotranslational, and N207 glycosylation of Kv1.1 occurs but is not required for subunit assembly, transport, or function.
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