What question did this study set out to answer?

To determine how progesterone regulates the expression of the c-jun protooncogene in the avian oviduct and evaluate its promoter responsiveness.

November 1, 1993Endocrinology

Rapid down-regulation of c-jun protooncogene transcription by progesterone in the avian oviduct.

View Full Paper

Ask AI

Bookmark

Authors

MSMalayannan SubramaniamSri Sathya Sai Institute of Higher Learning SHSteven A. HarrisPacific University Oregon KRKay RasmussenMayo Clinic

Discussion

Member takes

Implication

Experimental study demonstrates rapid down-regulation of c-jun transcription by progesterone in avian cells, suggesting negative regulatory elements within the gene promoter.

Key Points

To determine how progesterone regulates the expression of the c-jun protooncogene in the avian oviduct and evaluate its promoter responsiveness.
Administered progesterone to estrogen-withdrawn chickens and measured steady-state mRNA levels and nuclear run-off transcription rates over time.
Cotransfected transformed avian liver cells with progesterone receptor vectors and a chloramphenicol acetyltransferase reporter linked to the c-jun promoter (-1000 to +192).
Progesterone induced a dose-dependent decrease in c-jun mRNA within 30 minutes, decreasing the nuclear transcription rate by more than 80% within 15 minutes.
Ovalbumin transcription increased only after a 4-hour lag, while progesterone decreased reporter gene expression driven by the c-jun promoter construct.

Cite This Study

Subramaniam et al. (1993) studied this question.

synapsesocial.com/papers/6a0dabc468ddba849a09d2eb https://doi.org/10.1210/endo.133.5.8404652

View Full Paper

Ask AI

Bookmark

November 1, 1993Endocrinology

Rapid down-regulation of c-jun protooncogene transcription by progesterone in the avian oviduct.

View Full Paper

Ask AI

Bookmark

Authors

MSMalayannan SubramaniamSri Sathya Sai Institute of Higher Learning SHSteven A. HarrisPacific University Oregon KRKay RasmussenMayo Clinic

Discussion

Member takes

Implication

Experimental study demonstrates rapid down-regulation of c-jun transcription by progesterone in avian cells, suggesting negative regulatory elements within the gene promoter.

Key Points

To determine how progesterone regulates the expression of the c-jun protooncogene in the avian oviduct and evaluate its promoter responsiveness.
Administered progesterone to estrogen-withdrawn chickens and measured steady-state mRNA levels and nuclear run-off transcription rates over time.
Cotransfected transformed avian liver cells with progesterone receptor vectors and a chloramphenicol acetyltransferase reporter linked to the c-jun promoter (-1000 to +192).
Progesterone induced a dose-dependent decrease in c-jun mRNA within 30 minutes, decreasing the nuclear transcription rate by more than 80% within 15 minutes.
Ovalbumin transcription increased only after a 4-hour lag, while progesterone decreased reporter gene expression driven by the c-jun promoter construct.

Cite This Study

Subramaniam et al. (1993) studied this question.

synapsesocial.com/papers/6a0dabc468ddba849a09d2eb https://doi.org/10.1210/endo.133.5.8404652

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark