What question did this study set out to answer?

To examine the molecular mechanisms by which interleukin-6 and its receptor interact with the gp130 signal-transducing protein to activate intracellular tyrosine kinase signaling.

June 18, 1993Science

IL-6-Induced Homodimerization of gp130 and Associated Activation of a Tyrosine Kinase

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Authors

MMMasaaki MurakamiHokkaido University of Science MHMasahiko HibiNagoya University NNNaoko NakagawaKyoto University

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Implication

Mechanistic study demonstrates IL-6 induces gp130 homodimerization to activate tyrosine kinase, indicating a general receptor pairing mechanism in cytokine signaling.

Key Points

To examine the molecular mechanisms by which interleukin-6 and its receptor interact with the gp130 signal-transducing protein to activate intracellular tyrosine kinase signaling.
Assessed gp130 complex formation and disulfide-linked homodimerization upon IL-6 binding to IL-6 receptor.
Measured tyrosine kinase activity associated with monomeric versus dimerized forms of gp130.
Generated cytoplasmic gp130 mutants substituting serine for proline residues at positions 656 and 658 to evaluate functional signaling requirements.
IL-6 binding to the IL-6 receptor specifically induced disulfide-linked homodimerization of gp130.
Intracellular tyrosine kinase activity was associated exclusively with dimerized gp130 complexes, remaining undetectable in monomeric gp130.
Substitution of Pro656 and Pro658 with serine in the gp130 cytoplasmic motif abolished tyrosine kinase activation and cellular responses without disrupting gp130 homodimerization.

Cite This Study

Murakami et al. (1993) studied this question.

synapsesocial.com/papers/6a0dadc8e51d8d6d0c09cb60 https://doi.org/10.1126/science.8511589

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Implication

Mechanistic study demonstrates IL-6 induces gp130 homodimerization to activate tyrosine kinase, indicating a general receptor pairing mechanism in cytokine signaling.

IL-6-Induced Homodimerization of gp130 and Associated Activation of a Tyrosine Kinase

Discussion

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Implication

Key Points

Cite This Study

IL-6-Induced Homodimerization of gp130 and Associated Activation of a Tyrosine Kinase

Discussion

Member takes

Implication

Key Points

Cite This Study

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