What question did this study set out to answer?

To develop a sensitive immunoassay for measuring insulin levels based on competitive antibody precipitation.

May 1, 1962Experimental Biology and Medicine

Immunoassay of Insulin Using a Two-Antibody System.

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Authors

CMC. R. MorganIndiana University School of Medicine ALArnold LazarowUniversity of Minnesota

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Implication

Laboratory assay development demonstrates sensitive quantification of insulin using a two-antibody precipitation technique, enabling precise hormone detection below one microunit.

Key Points

To develop a sensitive immunoassay for measuring insulin levels based on competitive antibody precipitation.
Employed radioactive iodine-131 labeled insulin (I131-insulin) as a tracer precipitated by specific anti-insulin antibodies.
Added increasing quantities of unlabeled insulin to induce competitive displacement and alter precipitation percentages.
Demonstrated that the percentage of precipitated radioiodinated insulin systematically decreases as the concentration of unlabeled insulin increases.
Established an assay analytical sensitivity threshold capable of detecting less than one microunit of insulin.

Cite This Study

Morgan et al. (1962) studied this question.

synapsesocial.com/papers/6a0dae1acecdf5fb20ba8ddd https://doi.org/10.3181/00379727-110-27411

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May 1, 1962Experimental Biology and Medicine

Immunoassay of Insulin Using a Two-Antibody System.

View Full Paper

Ask AI

Bookmark

Authors

CMC. R. MorganIndiana University School of Medicine ALArnold LazarowUniversity of Minnesota

Discussion

Member takes

Implication

Laboratory assay development demonstrates sensitive quantification of insulin using a two-antibody precipitation technique, enabling precise hormone detection below one microunit.

Key Points

To develop a sensitive immunoassay for measuring insulin levels based on competitive antibody precipitation.
Employed radioactive iodine-131 labeled insulin (I131-insulin) as a tracer precipitated by specific anti-insulin antibodies.
Added increasing quantities of unlabeled insulin to induce competitive displacement and alter precipitation percentages.
Demonstrated that the percentage of precipitated radioiodinated insulin systematically decreases as the concentration of unlabeled insulin increases.
Established an assay analytical sensitivity threshold capable of detecting less than one microunit of insulin.

Cite This Study

Morgan et al. (1962) studied this question.

synapsesocial.com/papers/6a0dae1acecdf5fb20ba8ddd https://doi.org/10.3181/00379727-110-27411

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