What question did this study set out to answer?

To determine whether benign primitive hematopoietic progenitor cells can be distinguished and separated from malignant cells in chronic myelogenous leukemia bone marrow using cell-surface antigens.

February 15, 1992BloodOpen Access

Selection of benign primitive hematopoietic progenitors in chronic myelogenous leukemia on the basis of HLA-DR antigen expression

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To determine whether benign primitive hematopoietic progenitor cells can be distinguished and separated from malignant cells in chronic myelogenous leukemia bone marrow using cell-surface antigens.
Fractionated bone marrow cells expressing the CD34 surface antigen into HLA-DR-positive and HLA-DR-negative subpopulations.
Initiated long-term bone marrow cultures (LTBMCs) and evaluated secondary myeloid clonogenic progeny derived at weeks 1, 5, and 8.
Assayed clonogenic progeny for the Philadelphia chromosome (Ph1) and bcr/abl mRNA transcripts.
Primitive CD34+ progenitors lacking HLA-DR generated clonogenic progeny across weeks 1, 5, and 8 that lacked both the Philadelphia chromosome and bcr/abl mRNA.
Progenitors expressing HLA-DR (CD34+ HLA-DR+) generated clonogenic progeny that consistently retained the Philadelphia chromosome and expressed bcr/abl mRNA across all time points.

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Implication

In vitro study demonstrates separation of benign stem cells using HLA-DR expression in chronic myelogenous leukemia, suggesting a strategy for autologous bone marrow transplantation.

Cite This Study

Verfaillie et al. (1992) studied this question.

synapsesocial.com/papers/6a0db4ed68ddba849a09d92b https://doi.org/10.1182/blood.v79.4.1003.1003

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Implication

In vitro study demonstrates separation of benign stem cells using HLA-DR expression in chronic myelogenous leukemia, suggesting a strategy for autologous bone marrow transplantation.

Selection of benign primitive hematopoietic progenitors in chronic myelogenous leukemia on the basis of HLA-DR antigen expression

Key Points

Discussion

Implication

Cite This Study

Selection of benign primitive hematopoietic progenitors in chronic myelogenous leukemia on the basis of HLA-DR antigen expression

Key Points

Discussion

Implication

Cite This Study

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