The sequence of cDNA encoding lipoprotein lipase. A member of a lipase gene family.
Key result
The cDNA sequence of mature lipoprotein lipase reveals extensive homology with rat hepatic and porcine pancreatic lipases, indicating they belong to a highly conserved gene family of lipases.
Population
Mouse macrophage library, and tissues from mouse, human, bovine, and rat species
Comparison
cDNA cloning, sequencing, and mRNA expression… vs Nucleotide and amino acid sequences of rat…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Highlights conserved lipase gene family; leaves open human functional and therapeutic relevance.
Key Points
- The aim is to identify and analyze the cDNA for lipoprotein lipase and its evolutionary relationships within a gene family.
- Antibody screening of a mouse macrophage library to identify cDNA clones.
- Sequencing of the entire coding region of lipoprotein lipase.
- Comparison of sequences from mouse, rat, and porcine enzymes.
- Lipoprotein lipase consists of 447 amino acids with a molecular weight of 50,314.
- Significant homology was found with rat hepatic lipase and porcine pancreatic lipase, indicating a shared gene family.
- The mRNA for lipoprotein lipase is predominantly found in heart and adipose tissues, with major species sizes of 3.6 kb and 3.4 kb.
Structured PICO
P
Population
Mouse macrophage library, and tissues from mouse, human, bovine, and rat species
I
Intervention
cDNA cloning, sequencing, and mRNA expression analysis of lipoprotein lipase
C
Comparator
Nucleotide and amino acid sequences of rat hepatic lipase and porcine pancreatic lipase
O
Outcome
Nucleotide and predicted amino acid sequence, structural homology, and mRNA expression distribution
The identification of the lipoprotein lipase cDNA sequence reveals its membership in a highly conserved gene family of lipases with shared structural features.
Cite This Study
Kirchgessner et al. (1987) studied this question. The cDNA sequence of mature lipoprotein lipase reveals extensive homology with rat hepatic and porcine pancreatic lipases, indicating they belong to a highly conserved gene family of lipases.