Case report identifies canine circovirus associated with severe hemorrhagic diarrhea in a dog, highlighting the emergence of novel viral enteric pathogens.
Key Points
To investigate the clinical, pathological, and genomic characteristics of canine circovirus in a dog presenting with persistent hemorrhagic diarrhea.
Examined a 5-year-old male Golden Retriever presenting with acute watery and bloody diarrhea lasting three weeks.
Conducted histopathological analysis and polymerase chain reaction testing on small intestine, mesenteric lymph node, and fecal samples.
Sequenced the complete viral capsid gene and performed phylogenetic analysis against known reference strains.
Histopathological analysis revealed granulomatous inflammation, fibrinoid vasculitis, and intracytoplasmic amphophilic inclusion bodies in histiocytic cells within enteric tissues.
Polymerase chain reaction confirmed the presence of canine circovirus in small intestinal tissue, mesenteric lymph nodes, and feces.
Capsid gene sequencing demonstrated 94.2% nucleotide and 94.1% amino acid identity with the American isolate UCD3-478, classifying the virus as type 4 canine circovirus.