What question did this study set out to answer?

Identify small molecule inhibitors targeting conditionally essential enzymes within the wall teichoic acid biosynthetic pathway in Staphylococcus aureus.

August 18, 2009ACS Chemical BiologyOpen Access

Discovery of a Small Molecule that Blocks Wall Teichoic Acid Biosynthesis in Staphylococcus aureus

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Authors

JSJonathan G. SwobodaCenter for Biologics Evaluation and Research TMTimothy C. MeredithPennsylvania State University JCJennifer CampbellPublic Health Agency of Canada

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Implication

In vitro screen reveals a small molecule inhibitor of TarG that blocks cell wall biosynthesis in Staphylococcus aureus, highlighting a novel antibacterial strategy against resistant strains.

Key Points

Identify small molecule inhibitors targeting conditionally essential enzymes within the wall teichoic acid biosynthetic pathway in Staphylococcus aureus.
Developed a cell-based, pathway-specific screen that identifies small molecules inhibiting wild-type bacterial growth without affecting mutants defective in initiating polymer synthesis.
Screened for inhibitors of wall teichoic acid biosynthesis and characterized the target using combined genetic and biochemical approaches in clinical methicillin-resistant S. aureus isolates.
Discovered the small molecule 1835F03, which inhibited the growth of diverse S. aureus strains, including clinical MRSA isolates, at minimum inhibitory concentrations of 1–3 µg/mL.
Identified TarG, the transmembrane component of the ABC transporter that exports wall teichoic acids to the cell surface, as the molecular target whose inhibition triggers bacterial growth arrest.

Cite This Study

Swoboda et al. (2009) studied this question.

synapsesocial.com/papers/6a0df2fccae7912d2fa56cc3 https://doi.org/10.1021/cb900151k

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Implication

In vitro screen reveals a small molecule inhibitor of TarG that blocks cell wall biosynthesis in Staphylococcus aureus, highlighting a novel antibacterial strategy against resistant strains.

Discovery of a Small Molecule that Blocks Wall Teichoic Acid Biosynthesis in Staphylococcus aureus

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Implication

Key Points

Cite This Study

Discovery of a Small Molecule that Blocks Wall Teichoic Acid Biosynthesis in Staphylococcus aureus

Discussion

Member takes

Implication

Key Points

Cite This Study

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