What question did this study set out to answer?

To review the major cell engineering approaches used to optimize mammalian host cells and evaluate their impact on bioprocess productivity and product quality.

April 1, 2010Biotechnology and Applied Biochemistry

Engineering mammalian cells in bioprocessing – current achievements and future perspectives

View Full Paper

Ask AI

Bookmark

Authors

YLYiping Lim NWNiki S.C. WongBioprocessing Technology Institute YLYih Yean LeeThe University of Melbourne

Discussion

Member takes

Implication

Review highlights cell engineering strategies that enhance protein titers in mammalian bioprocessing systems, indicating genetic targets for optimizing metabolism, secretion, and cell survival.

Key Points

To review the major cell engineering approaches used to optimize mammalian host cells and evaluate their impact on bioprocess productivity and product quality.
Surveyed genetic engineering strategies altering mammalian host cell metabolism, cell cycle regulation, protein secretion, and apoptosis pathways.
Evaluated recombinant DNA interventions aimed at improving post-translational modifications, specifically antibody glycosylation and sialylation.
Bioprocess product titers improved more than 100-fold over two decades, increasing from 50 mg/L to 5–10 g/L through bioprocess optimization and host cell engineering.
Metabolic and cell cycle modifications successfully reduced toxic waste metabolite accumulation and enabled biphasic production systems.
Targeting anti-apoptosis genes extended culture viability, while manipulating endoplasmic reticulum chaperones and glycosylation enzymes improved protein secretion and therapeutic efficacy.

Cite This Study

Lim et al. (2010) studied this question.

synapsesocial.com/papers/6a0e1e46e56f987c96d08bd4 https://doi.org/10.1042/ba20090363

View Full Paper

Ask AI

Bookmark

Implication

Review highlights cell engineering strategies that enhance protein titers in mammalian bioprocessing systems, indicating genetic targets for optimizing metabolism, secretion, and cell survival.

Engineering mammalian cells in bioprocessing – current achievements and future perspectives

Discussion

Member takes

Implication

Key Points

Cite This Study

Engineering mammalian cells in bioprocessing – current achievements and future perspectives

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider