Key result
A rapid RT-PCR and microwell capture hybridization assay detected enteroviral RNA in 38.2% of explanted hearts from patients with chronic dilated cardiomyopathy, compared to 34.5% by nested RT-PCR.
Why the study?
Does a rapid RT-PCR and hybridization assay improve the detection of enteroviral RNA in endomyocardial tissue from patients with chronic dilated cardiomyopathy compared to nested RT-PCR and cell culture?
Observational (n=110)
Does a rapid RT-PCR and hybridization assay improve the detection of enteroviral RNA in endomyocardial tissue from patients with chronic dilated cardiomyopathy compared to nested RT-PCR and cell culture?
Absolute Event Rate: 38.2% vs 34.5%
A rapid RT-PCR and hybridization assay effectively detects enteroviral RNA in endomyocardial tissue, supporting the persistence of group A and B Coxsackieviruses in end-stage chronic dilated cardiomyopathy.
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Supports viral persistence in chronic DCM; hypothesis-generating for rapid assay in endomyocardial analysis.
Rey et al. (2001) conducted an observational in Chronic dilated cardiomyopathy (n=110). Rapid RT-PCR and microwell capture hybridisation assay vs. Nested RT-PCR and classical cell culture assays was evaluated on Detection of enteroviral RNA sequences in endomyocardial tissue. A rapid RT-PCR and microwell capture hybridization assay detected enteroviral RNA in 38.2% of explanted hearts from patients with chronic dilated cardiomyopathy, compared to 34.5% by nested RT-PCR.
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