Regional diastolic dysfunction in post-infarction heart failure: role of local mechanical load and SERCA expression

Figures

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  Figure 1. Slowing of relaxation and elevated wall stress in the adjacent region of HFrEF rats. MRI images illustrate the division of HFrEF rat hearts into three regions (adjacent, medial, and remote) according to their proximity to the infarction (A). Comparison was made to equivalent regions in sham-operated controls. Representative recordings (B) and mean data (C) revealed lower peak circumferential systolic strain adjacent to the infarct in HFrEF. The rate of relaxation was dyssynchronous across the failing heart, as indicated by measurements of circumferential strain rate (D). Specifically, peak diastolic strain rate was only reduced in the adjacent region of HFrEF hearts, and was increased in the remote region (E). Wall stress was calculated across sham and HFrEF hearts based on intraventricular pressure measurements and local ventricular geometry (see Methods section). Measurements are presented as bullseye plots (F) and tracings of wall stress values during the cardiac cycle (G). Mean data demonstrate that while integrated wall stress was high in all regions of HFrEF hearts, values were particularly elevated in the adjacent region (H). (nhearts = 14, 29 in sham, HFrEF). *P < 0.05…

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  Figure 2. Impaired diastolic Ca2+ handling in cardiomyocytes from the adjacent region. Representative recordings of whole-cell, wide-field Ca2+ transients (A, 1 Hz and 6 Hz stimulation, fluo-4), and mean data (B) revealed that, relative to sham, Ca2+ transient magnitude was maintained in cardiomyocytes isolated from the adjacent zone of HFrEF hearts, but reduced in the medial and remote zones. However, HFrEF cells from the adjacent region exhibited significantly slower Ca2+ transient decay (C), and at high-pacing frequencies, a significant elevation of diastolic Ca2+ levels (D). (nhearts = 4, 5 in sham, HFrEF). Comparisons between HFrEF regions are presented in Supplementary material online, Figure S1. *P < 0.05 vs. sham calculated with nested ANOVA.

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  Figure 3. Reduced SERCA activity in the adjacent region. Representative recordings of Ca2+ transients are illustrated for cardiomyocytes during 1 Hz pacing, followed by rapid application of 10 mmol/L caffeine (A). The magnitude of caffeine-elicited Ca2+ release, an indicator of SR Ca2+ content, tended to be lower in the medial and distal regions (B). Fits of the declining phases of 1 Hz and caffeine transients revealed slowed SR Ca2+ reuptake in HFrEF cardiomyocytes from the adjacent region (C), while the rate of NCX Ca2+ extrusion tended to be higher in the remote zone (D) (nhearts = 4, 5 in sham, HFrEF). (E) Caffeine transients recorded in the presence of 5 mmol/L Ni2+ revealed no alterations in Ca2+ fluxes via slow extrusion pathways (nhearts = 2, 3 in sham, HFrEF). (F) Simulations of Ca2+ transients at physiological frequency (6 Hz) demonstrate the consequences of enhancing NCX activity (blue), as observed in myocytes from the distal zone, or reducing SERCA activity (red), as observed in the adjacent zone. *P < 0.05, calculated by nested ANOVA.

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  Figure 4. Lower SERCA2 expression in the adjacent region. Consistent with impaired SERCA function in the adjacent region, representative immunoblots (A, vinculin as loading control), and mean data (B) show that SERCA2 expression was significantly reduced in the adjacent region. By contrast, expression of phospholamban (PLB, C), NCX (D), the L-type Ca2+ channel (Cav1.2, E), and the ryanodine receptor (RyR, F) were not markedly altered across HFrEF and sham hearts. Complete blots are presented in Supplementary material online, Figure S4 (nhearts = 6, 6 in sham, HFrEF). *P < 0.05, calculated by two-way ANOVA with a post hoc Bonferroni t-test.

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  Figure 5. High mechanical stress down-regulates SERCA2 and slows relaxation in ex vivo myocardial tissue. Excised rat papillary muscles were stretched to reproduce high wall stress values comparable to those in the adjacent region of HFrEF hearts, and maintained during 48 h of culture. Comparison was made with muscles subjected to low wall stress conditions approximating those present in the normal heart. Average tension during the protocol is illustrated in A for representative muscles. High stress triggered a significant down-regulation of SERCA2 mRNA (B) (nmuscles = 4 in low group, 3 in high). Returning high stress muscles to normal levels at the completion of the protocol revealed that SERCA2 down-regulation was associated with marked slowing of force decline (representative recordings in C; mean data in D) (nmuscles = 11, 9 in low, high). *P < 0.05 vs. low stress calculated with Student’s t-test.

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  Figure 6. Selection of LEAF trial18 participants. HFrEF patients were compared with non-failing ‘control’ individuals (EF ≥50%) who were without a detectable myocardial infarction at 6 weeks of follow-up.

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  Figure 7. HFrEF patients exhibited regional diastolic dysfunction adjacent to the infarction. Four-chamber, apical echocardiographic images of HFrEF patients were examined to divide the viable myocardium into regions adjacent and remote to the myocardial infarction (schematically illustrated in A, right panel). Using speckle-tracking to examine local strain at indicated regions (arrows), comparison was made with regionally matched values in control hearts (A, left panel). Peak strain was reduced in HFrEF hearts, particularly in the adjacent region (B). Representative recordings of strain rate (C) and mean measurements (D) reveal that diastolic strain rate was also reduced adjacent to the myocardial infarction in HFrEF, but similar to control values in the remote region (nhearts = 12, 9 in control, HFrEF). *P < 0.05 with two-way ANOVA with a post hoc Bonferroni t-test.