Algoriphagus machipongonensis sp. nov., co-isolated with a colonial choanoflagellate

A Gram-negative, non-motile, non-spore-forming bacterial strain, PR1 T , was isolated from a mud core sample containing colonial choanoflagellates near Hog Island, Virginia, USA. Strain PR1 T grew optimally at 30 °C and with 3 % (w/v) NaCl. Strain PR1 T contained MK-7 as the major menaquinone as well as carotenoids but lacked pigments of the flexirubin-type. The predominant fatty acids were iso-C 15 : 0 (29.4 %), iso-C 17 : 1 ω9 c (18.5 %) and summed feature 3 (C 16 : 1 ω6 c and/or C 16 : 1 ω7 c ; 11.3 %). The major polar lipids detected in strain PR1 T were phosphatidylethanolamine, an unknown phospholipid, an aminophospholipid, an aminolipid and two lipids of unknown character. The DNA G+C content was 38.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PR1 T fell within the cluster comprising the genus Algoriphagus and was most closely related to Algoriphagus halophilus JC 2051 T (95.4 % sequence similarity) and Algoriphagus lutimaris S1-3 T (95.3 % sequence similarity). The 16S rRNA gene sequence similarity between strain PR1 T and the type strains of other species of the genus Algoriphagus were in the range 91–95 %. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain PR1 T demonstrated that this strain was distinct from other members of the genus Algoriphagus , including its closest relative, A. halophilus . Based on phenotypic, chemotaxonomic, phylogenetic and genomic data, strain PR1 T should be placed in the genus Algoriphagus as a representative of a novel species, for which the name Algoriphagus machipongonensis sp. nov. is proposed. The type strain is PR1 T ( = ATCC BAA-2233 T = DSM 24695 T ).