Key result
The Thrombogram method enables continuous, direct monitoring of thrombin activity in platelet-rich plasma using a fluorescent substrate that is not impaired by clot turbidity.
Describes a novel method for continuous monitoring of thrombin generation in platelet-rich plasma using a fluorescent substrate, overcoming limitations of turbidity.
May enable continuous thrombin monitoring in platelet-rich plasma; leaves open clinical validation before diagnostic adoption.
A method is described in which thrombin activity in clotting plasma can be monitored through the continuous measurement of the fluorescent split-product of the substrate Z-Gly-Gly-Arg-AMC. The signal is not impaired by turbidity; therefore proper measurement is not disturbed by the occurrence of a clot or the presence of platelets and direct measurement in platelet rich plasma is possible.
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Giesen et al. (2000) studied this question. The Thrombogram method enables continuous, direct monitoring of thrombin activity in platelet-rich plasma using a fluorescent substrate that is not impaired by clot turbidity.
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