Cardiac optical mapping – State-of-the-art and future challenges

Figures

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  Fig. 1. Cardiac optical mapping setup, data and analysis. A) Schematic representation of a typical optical mapping setup for imaging a potentiometric dye loaded cardiac preparation (left). Inset shows the fluorescent indicator embedded within the cellular membrane. The potentiometric dye is excited by photons (green arrows) from an illumination source. This causes the release of fluorescent photons (red arrows) whose spectral properties depend on the transmembrane voltage. Fluorescent photons are filtered from the illumination photons and directed to a high-density imaging detector to produce a time series dataset. B) Example of analyses possible from optical mapping datasets, including activation and signal morphology mapping (left panel). Right panel shows examples of the raw signals produced at each pixel of an optical mapping dataset, in this case optical action potentials from mouse ventricles. Y axis denotes normalised fractional fluorescence change (F/F0) from baseline fluorescence level (F0). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).

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  Fig. 2. Challenges in cardiac optical mapping. A) Example of processing techniques that are often applied on all pixels in an optical mapping dataset to enhance the signal to noise ratio of the raw data (left) to allow effective parameter quantification (right). Note: The exact methods and sequence of processing methods used will vary depending on experimental setup, model and analysis software used. B) Schematic representation of a typical imaged area (green) from a mouse whole heart using a single camera setup. The red area shows the area of the epicardial ventricular surface that is not imaged. C) Example signals from a mouse atrium where motion artefacts are present. In the area with prolonged APD (red), contraction has not been successfully uncoupled, distorting the measured optical signal. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).