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June 19, 2017Clinical ProteomicsOpen Access

A novel workflow combining plaque imaging, plaque and plasma proteomics identifies biomarkers of human coronary atherosclerotic plaque disruption

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Key result

PCI-induced plaque disruption is linked to a ~44% increase in LBP, a novel plaque rupture biomarker.

  • 1.44 times increase
  • 95% CI 1.07-1.81
  • P=0.0159
  • n=81

Why the study?

Does a workflow combining plaque imaging and proteomics identify biomarkers of human coronary atherosclerotic plaque disruption?

Population

Humans undergoing angioplasty-induced plaque disruption

Comparison

Plaque imaging combined with plaque and plasma… vs Corresponding control samples

Design

Other

Follow-up

5 minutes post-disruption

Authors

RLRegent LeeRFRomán FischerPCPhilip D. Charles

Discussion

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Overview

LBP rise may flag plaque rupture during PCI; extends OCT-proteomics workflows for coronary biomarker discovery.

Study Design

Type

Observational (n=81)

Multicenter

No

Structured PICO

Does a workflow combining plaque imaging and proteomics identify biomarkers of human coronary atherosclerotic plaque disruption?

P
Population
Humans undergoing angioplasty-induced plaque disruption
I
Intervention
Plaque imaging (optical coherence tomography) combined with plaque and plasma proteomic analysis (LC-MS/MS)
C
Comparator
Corresponding control samples
O
Outcome
Identification of biomarkers of human coronary atherosclerotic plaque disruptionsurrogate

Main Result

Effect estimate: 1.44 times increase (95% CI 1.07-1.81)

p-value: p=0.0159

A novel workflow integrating optical coherence tomography and proteomics identified new biomarkers, including MMP9 and five novel proteins, associated with human coronary plaque disruption.

Limitations

  • Small sample size requiring further validation in larger cohorts to improve external validity
  • Need to establish the time course profile of these proteins and their physiological concentration ranges in large cohorts

Cite This Study

Lee et al. (2017) conducted an observational in Coronary atherosclerosis (n=81). Percutaneous coronary intervention (PCI) vs. Diagnostic angiography without stenting was evaluated on Plasma lipopolysaccharide binding protein (LBP) levels (1.44 times increase, 95% CI 1.07-1.81, p=0.0159). Percutaneous coronary intervention-induced plaque disruption led to a 1.44-fold increase in systemic lipopolysaccharide binding protein levels, identifying it as a novel biomarker of plaque rupture.

synapsesocial.com/papers/6a0f76802badbc352afe345chttps://doi.org/10.1186/s12014-017-9157-x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Update on acute coronary syndromes: the pathologists' view2012 · 1,059 citations
  2. 2High Levels of Systemic Myeloperoxidase Are Associated With Coronary Plaque Erosion in Patients With Acute Coronary Syndromes2010 · 236 citations
  3. 3Enhanced Inflammatory Response to Coronary Angioplasty in Patients With Severe Unstable Angina1998 · 308 citations
  4. 4Impact of Coronary Plaque Morphology Assessed by Optical Coherence Tomography on Cardiac Troponin Elevation in Patients With Elective Stent Implantation2011 · 109 citations
  5. 5The consequences of sample pooling in proteomics: An empirical study2009 · 209 citations