A rapid, sensitive, and reproducible method for the assay of lipoprotein lipase was developed using a stable, radioactive substrate emulsion of fatty acid-labeled trioleoylglycerol.
The study describes a rapid, sensitive, and reproducible method for assaying lipoprotein lipase using a stable radioactive substrate emulsion.
A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use.
Nilsson‐Ehle et al. (1976) studied this question. Radioactive substrate emulsion assay for lipoprotein lipase was evaluated. A rapid, sensitive, and reproducible method for the assay of lipoprotein lipase was developed using a stable, radioactive substrate emulsion of fatty acid-labeled trioleoylglycerol.