What question did this study set out to answer?

To develop and validate an artificial liposome substrate system for the sensitive and reproducible measurement of serum lecithin:cholesterol acyltransferase (LCAT) activity.

October 1, 1987Journal of Lipid ResearchOpen Access

New substrate for determination of serum lecithin:cholesterol acyltransferase

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To develop and validate an artificial liposome substrate system for the sensitive and reproducible measurement of serum lecithin:cholesterol acyltransferase (LCAT) activity.
Screened liposomes formulated from cholesterol and diverse synthetic phosphatidylcholines, identifying dimyristoylphosphatidylcholine (DMPC) as the most reactive substrate.
Quantified LCAT activity across 120 human serum samples by enzymatically tracking unesterified cholesterol depletion and assessed assay linearity, recovery, and precision.
LCAT activity in 120 human sera averaged 485.4 ± 64.6 nmol/hr per ml (mean ± SD), representing a 4.4- to 5.4-fold increase over conventional self-substrate methods.
The assay demonstrated linear response up to 670 nmol/hr per ml, achieved coefficients of variation below 4%, and efficiently recovered activity when partially purified LCAT was added to heat-inactivated serum.

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Implication

Laboratory study demonstrates a sensitive liposome-based assay for measuring lecithin:cholesterol acyltransferase in human serum, indicating high reproducibility without matrix interference.

Cite This Study

Manabe et al. (1987) studied this question.

synapsesocial.com/papers/6ab6093711ffef926e20c3e5 https://doi.org/10.1016/s0022-2275(20)38610-7

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Implication

Laboratory study demonstrates a sensitive liposome-based assay for measuring lecithin:cholesterol acyltransferase in human serum, indicating high reproducibility without matrix interference.

New substrate for determination of serum lecithin:cholesterol acyltransferase

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Discussion

Implication

Cite This Study

New substrate for determination of serum lecithin:cholesterol acyltransferase

Key Points

Discussion

Implication

Cite This Study

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