What question did this study set out to answer?

To determine whether regulatory pathways beyond the canonical CBF/DREB1 cold response pathway coordinate cold acclimation and freezing tolerance in Arabidopsis thaliana.

August 1, 2002The Plant CellOpen Access

Arabidopsis Transcriptome Profiling Indicates That Multiple Regulatory Pathways Are Activated during Cold Acclimation in Addition to the CBF Cold Response PathwayW

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Authors

SFSarah FowlerMichigan State University MTMichael F. ThomashowMichigan State University

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Implication

Transcriptomic analysis reveals diverse CBF-independent regulatory pathways in Arabidopsis, indicating that freezing tolerance relies on complex multiple-network gene activation and repression.

Key Points

To determine whether regulatory pathways beyond the canonical CBF/DREB1 cold response pathway coordinate cold acclimation and freezing tolerance in Arabidopsis thaliana.
Measured transcript levels across approximately 8,000 genes at multiple time points during a 7-day cold exposure following transfer from warm to cold conditions.
Profiled warm-grown transgenic plants constitutively expressing CBF1, CBF2, or CBF3 to resolve CBF-dependent versus CBF-independent regulons.
Identified 306 cold-responsive genes displaying a threefold or greater change, with 218 genes upregulated and 88 genes downregulated.
Established that only 12% of cold-responsive genes are certain members of the CBF regulon, whereas at least 28% (including 15 transcription factors) function independently of CBF regulation.
Demonstrated that CBF expression in warm conditions repressed eight genes that are also downregulated by cold, indicating that transcriptional repression is integral to cold acclimation.

Cite This Study

Fowler et al. (2002) studied this question.

synapsesocial.com/papers/6a0fa99f8090e499da600694 https://doi.org/10.1105/tpc.003483

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Implication

Transcriptomic analysis reveals diverse CBF-independent regulatory pathways in Arabidopsis, indicating that freezing tolerance relies on complex multiple-network gene activation and repression.

Arabidopsis Transcriptome Profiling Indicates That Multiple Regulatory Pathways Are Activated during Cold Acclimation in Addition to the CBF Cold Response PathwayW

Discussion

Member takes

Implication

Key Points

Cite This Study

Arabidopsis Transcriptome Profiling Indicates That Multiple Regulatory Pathways Are Activated during Cold Acclimation in Addition to the CBF Cold Response PathwayW

Discussion

Member takes

Implication

Key Points

Cite This Study

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