What question did this study set out to answer?

To clone and characterize a full-length cDNA for human hypoxanthine phosphoribosyltransferase (HPRT).

Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.

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Randomized trial demonstrates expression of human HPRT enzyme in transfected cells, indicating potential for genetic therapies.

To clone and characterize a full-length cDNA for human hypoxanthine phosphoribosyltransferase (HPRT).
Cloning a 1.6-kilobase cDNA of human HPRT into a simian virus 40-based expression vector.
Determining the full nucleotide sequence of the cloned cDNA.
Transfecting HPRT-deficient mouse LA9 cells with the purified plasmid to assess enzyme activity.
The inferred amino acid sequence from the cDNA matches a previously identified partial sequence of authentic human HPRT protein.
Transfected LA9 cells successfully expressed human HPRT enzyme activity after selection in hypoxanthine/aminopterin/thymidine medium.

Jolly et al. (1983) studied this question.

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