What question did this study set out to answer?

To design and characterize group-specific 16S rRNA oligonucleotide hybridization probes capable of identifying and profiling natural communities of methanogens.

April 1, 1994Applied and Environmental MicrobiologyOpen Access

Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens

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Authors

LRLutgarde RaskinNorthwestern University JSJ. M. StromleyUniversity of Illinois Urbana-Champaign BRBruce E. RittmannUniversité de Technologie de Compiègne

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Implication

Molecular assay development demonstrates high target specificity of eight 16S rRNA probes in methanogens, indicating broad utility for microbial community profiling.

Key Points

To design and characterize group-specific 16S rRNA oligonucleotide hybridization probes capable of identifying and profiling natural communities of methanogens.
Designed eight oligonucleotide hybridization probes targeted to conserved regions of 16S rRNA across phylogenetically defined groups of methanogens.
Characterized the hybridization target-group specificity and the temperature of dissociation for each oligonucleotide probe against target and non-target methanogens.
The eight designed probes specifically bound their intended methanogen targets without cross-hybridizing to non-target methanogen rRNAs, circumscribing cultured methanogens except the family Methanothermaceae.
Three probes exhibited order-level specificity (two targeting Methanobacteriales and one targeting Methanococcales), while five probes differentiated families and genera within Methanomicrobiales and Methanosarcinaceae.

Cite This Study

Raskin et al. (1994) studied this question.

synapsesocial.com/papers/6a0fcb569e54838161fd338b https://doi.org/10.1128/aem.60.4.1232-1240.1994

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Implication

Molecular assay development demonstrates high target specificity of eight 16S rRNA probes in methanogens, indicating broad utility for microbial community profiling.

Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens

Discussion

Member takes

Implication

Key Points

Cite This Study

Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens

Discussion

Member takes

Implication

Key Points

Cite This Study

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