Key result
Optical histology combining chemical optical clearing and optical imaging increased the visualized functional vascular density of mouse cardiac microvasculature by 1.5 to 2.0 times compared to uncleared tissue.
Optical histology provides a novel method for high-resolution, wide-field imaging of the cardiac microvasculature in preclinical models.
Cardiovascular disease typically is associated with dysfunction of the coronary vasculature and microvasculature. The study of cardiovascular disease typically involves imaging of the large coronary vessels and quantification of cardiac blood perfusion. These methods, however, are not well suited for imaging of the cardiac microvasculature. We used the optical histology method, which combines chemical optical clearing and optical imaging, to create high-resolution, wide-field maps of the cardiac microvasculature in ventral slices of mouse heart. We have demonstrated the ability of the optical histology method to enable wide-field visualization of the cardiac microvasculature in high-resolution and anticipate that optical histology may have significant impact in studying cardiovascular disease.
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Moy et al. (2013) studied Healthy mouse cardiac microvasculature (n=9). Optical histology (FocusClear optical clearing) vs. Native (uncleared) tissue was evaluated on Functional vascular density (FVD). Optical histology combining chemical optical clearing and optical imaging increased the visualized functional vascular density of mouse cardiac microvasculature by 1.5 to 2.0 times compared to uncleared tissue.
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