What question did this study set out to answer?

Clone and characterize the cDNA of the 29-kDa subunit of the multicatalytic protease activator and assess its functional regulation by gamma-interferon.

August 1, 1994Journal of Biological ChemistryOpen Access

Molecular cloning and expression of a gamma-interferon-inducible activator of the multicatalytic protease.

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Authors

CRClaudio RealiniSwiss Institute for Regenerative Medicine WDWolfgang DubielOtto-von-Guericke University Magdeburg GPG PrattUniversity of Utah

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Implication

In vitro experimental study demonstrates cloning and interferon-gamma induction of a proteasome activator subunit, highlighting mechanisms of class I antigen presentation.

Key Points

Clone and characterize the cDNA of the 29-kDa subunit of the multicatalytic protease activator and assess its functional regulation by gamma-interferon.
Cloned and sequenced the cDNA encoding the 29-kDa subunit of the hexameric multicatalytic protease activator.
Expressed the recombinant protein in Escherichia coli and tested its binding, structural properties, and enzymatic stimulation against red blood cell-derived activator.
Assayed activator subunit synthesis in HeLa cells following exposure to gamma-interferon.
The cloned cDNA encoded a 249-amino-acid protein containing a 28-residue alternating lysine-glutamic acid sequence (KEKE motif) also found in 26S protease subunits and chaperonins.
Recombinant activator bound the multicatalytic protease, forming three isoelectric species and stimulating cleavage at the carboxyl terminus of hydrophobic or charged residues.
Synthesis of the activator subunit was selectively induced by gamma-interferon treatment in HeLa cells.

Cite This Study

Realini et al. (1994) studied this question.

synapsesocial.com/papers/6a0fe2cfd8c5cf602efd4b4e https://doi.org/10.1016/s0021-9258(17)32052-5

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Implication

In vitro experimental study demonstrates cloning and interferon-gamma induction of a proteasome activator subunit, highlighting mechanisms of class I antigen presentation.

Molecular cloning and expression of a gamma-interferon-inducible activator of the multicatalytic protease.

Discussion

Member takes

Implication

Key Points

Cite This Study

Molecular cloning and expression of a gamma-interferon-inducible activator of the multicatalytic protease.

Discussion

Member takes

Implication

Key Points

Cite This Study

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