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August 1, 1997Journal of Clinical MicrobiologyOpen Access

Multiplex reverse transcription-PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996

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Why the study?

Does multiplex RT-PCR improve the detection of influenza viruses compared to culture in patients with influenzalike illness?

Population

619 combined nose and throat swabs from individuals presenting with 'influenzalike illness' taken by general…

Comparison

Multiplex reverse transcription-PCR for… vs Culture and immunofluorescence

Design

Cross-sectional

Authors

JEJoanna EllisNational Security AgencyDFDouglas FlemingNational Health ServiceMZMaria ZambonNational Health Service

Discussion

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Implication

Supports higher influenza detection in surveillance; leaves open whether RT-PCR should replace culture in routine diagnostics.

Structured PICO

Does multiplex RT-PCR improve the detection of influenza viruses compared to culture in patients with influenzalike illness?

P
Population
619 combined nose and throat swabs from individuals presenting with 'influenzalike illness' taken by general practitioners in sentinel practices
I
Intervention
Multiplex reverse transcription-PCR (RT-PCR) for detection, typing, and subtyping of the hemagglutinin gene of influenza type A (H3N2 and H1N1) and type B viruses
C
Comparator
Culture and immunofluorescence
O
Outcome
Detection of influenza virusessurrogate

Multiplex RT-PCR provides a more sensitive and accurate method for influenza surveillance directly from clinical specimens compared to traditional culture.

Cite This Study

Ellis et al. (1997) studied this question.

synapsesocial.com/papers/6a0ff0d357bfcc72645fc616https://doi.org/10.1128/jcm.35.8.2076-2082.1997
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