What question did this study set out to answer?

To determine whether the Src-family tyrosine kinase p60fyn is enzymatically activated upon stimulation of the T cell antigen receptor.

September 1, 1992Journal of Biological ChemistryOpen Access

Activation of tyrosine kinase p60fyn following T cell antigen receptor cross-linking.

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Authors

ATAlexander Y. TsygankovTemple University BBBarbara M. BrökerUniversitätsmedizin Greifswald JFJoseph FargnoliBristol-Myers Squibb (Germany)

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Implication

Experimental study demonstrates selective activation of tyrosine kinase p60fyn via CD3 cross-linking in human T cells, indicating direct functional coupling to antigen receptor signaling.

Key Points

To determine whether the Src-family tyrosine kinase p60fyn is enzymatically activated upon stimulation of the T cell antigen receptor.
Stimulated human T cell models—including resting peripheral T cells, helper T cell clones, an immortalized helper clone, and a leukemic T cell line—using antibody-mediated cross-linking.
Evaluated enzymatic activation and phosphotyrosine content of p60fyn following cross-linking of CD3 compared with CD4, CD2, CD28, and assessed other Src-related kinases.
CD3 cross-linking rapidly induced a transient 2- to 4-fold increase in p60fyn enzymatic activity over basal levels, while total p60fyn protein abundance remained unchanged.
Activation of p60fyn was accompanied by an increase in its phosphotyrosine content and was elicited by anti-CD3 antibodies, but not by anti-CD4, anti-CD2, or anti-CD28 antibodies.
No other Src-related tyrosine kinases exhibited activation following CD3 cross-linking.

Cite This Study

Tsygankov et al. (1992) studied this question.

synapsesocial.com/papers/6a0ff2805725bbd5cc604a58 https://doi.org/10.1016/s0021-9258(19)36951-0

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Implication

Experimental study demonstrates selective activation of tyrosine kinase p60fyn via CD3 cross-linking in human T cells, indicating direct functional coupling to antigen receptor signaling.

Activation of tyrosine kinase p60fyn following T cell antigen receptor cross-linking.

Discussion

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Implication

Key Points

Cite This Study

Activation of tyrosine kinase p60fyn following T cell antigen receptor cross-linking.

Discussion

Member takes

Implication

Key Points

Cite This Study

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