A dual-band RF exposure system for real-time electrophysiological recordings using microelectrode array device

Introduction Fifth-generation (5G) wireless communication systems operating in the FR1 band have increased interest in studying bioelectromagnetic interactions under controlled exposure conditions. MicroElectrode Array (MEA)-based electrophysiology provides a suitable approach for real-time investigations of neuronal activity, although its integration with RF exposure systems remains technically challenging. Methods This work presents the design, integration, and numerical characterization of a dual-band RF exposure system for real-time electrophysiological recordings using MEA devices. The system operates at 0.7 GHz and 3.5 GHz and is based on an open-ended coaxial radiator, termed Localized Radiating-End Coaxial line (LoREC). Full-wave COMSOL simulations were performed to evaluate field confinement, dosimetric quantities (SAR), and compatibility with the MEA acquisition setup. Results The proposed exposure system achieved stable impedance matching and effective confinement of the electromagnetic field within the biological target region. SAR analysis showed frequency-dependent exposure patterns with reproducible and spatially consistent distributions across the active electrode area. Simulations also demonstrated negligible residual fields in proximity to the acquisition electronics, indicating limited electromagnetic interference with electrophysiological recordings. Discussion The proposed exposure system provides a controlled and reproducible exposure architecture suitable for real-time RF exposure and MEA electrophysiological measurements under physiological conditions. The numerical results demonstrated stable near-field confinement within the MEA culture region, predictable SAR distributions, and negligible residual fields on the acquisition electronics across the investigated frequency range. The system is therefore suitable for investigating frequency-dependent bioelectromagnetic effects associated with 5G-FR1 technologies.