What question did this study set out to answer?

This research aims to improve UDP-glucose regeneration for glycosylation processes using thermophilic enzymes.

Development of a UDP-Glucose Regeneration Cascade Using Thermophilic Enzymes

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Randomized trial developed UDP-glucose regeneration system in vitro, suggesting improved glycosylation efficiency.

This research aims to improve UDP-glucose regeneration for glycosylation processes using thermophilic enzymes.
Developed a UDP-glucose regeneration cascade based on non-oxidative glycolysis
Utilized phosphorolysis of maltodextrin for energy and glucose
Employed thermostable enzymes for one-step enzyme preparation
Achieved a conversion yield of 98.1 mol % for trehalose synthesis from glucose
Eliminated the need for excess sacrificial substrates
Demonstrated strong equilibrium bias toward UDP-glucose formation

Suzuki et al. (2026) studied this question.

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