Randomized trial investigates neuroinflammation caused by P.g-OMVs in a mouse model, suggesting new therapeutic targets.
Key Points
This study aims to explore how Porphyromonas gingivalis outer membrane vesicles influence neuroinflammation and mitochondrial dysfunction in Alzheimer's disease.
C57BL/6 mice received bilateral gingival injections of P.g-OMVs three times weekly for eight weeks.
In vitro HT22 cells were treated with P.g-OMVs to measure inflammatory cytokines and TGM2 expression.
RNA-seq was used to identify differentially expressed genes in brain tissues of treated mice.
P.g-OMVs caused significant cognitive impairment in mice, evident by escape latency (p < 0.001) and increase in amyloid β and hyperphosphorylated tau.
In vitro studies showed increased pro-inflammatory cytokines and upregulation of TGM2 (p < 0.05).
TGM2 knockdown reversed inflammation and mitochondrial dysfunction associated with MAM formation.