Integrated high‐throughput phenomics and transcriptomics uncover the transcriptional mechanisms underlying rice responses to elevated CO 2 concentration and cadmium stress
Randomized trial uncovers transcriptional responses to cadmium stress and elevated CO2 in rice, indicating pathways for breeding low-cadmium varieties.
Key Points
The research aims to identify the genetic and transcriptional responses of rice to cadmium stress and elevated carbon dioxide levels.
High-throughput phenotyping of rice cultivar Nipponbare across six developmental stages under cadmium stress and three elevated CO2 concentrations.
Multi-tissue transcriptome analyses to identify differentially expressed genes related to stress responses.
Construction of an integrative co-expression network highlighting stress-responsive subnetworks.
Identification of Multiple Stress Responsive Gene 3 (OsMSR3) as a crucial hub gene for Cd and eCO2 responses.
Observation of decreasing haplotype 2 (Hap2) frequency of OsMSR3 from low- to high-latitude regions in China, correlating with Cd-contaminated soil distribution.
Revealed a transcriptional regulatory network that provides insights for breeding rice with low cadmium accumulation.