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This study aims to identify hub genes and their regulatory mechanisms during cervical cancer progression using fresh tissue samples.

May 22, 2026PeerJOpen Access

Multi-stage transcriptome analysis identifies hub genes and regulatory mechanisms driving cervical cancer progression

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This study aims to identify hub genes and their regulatory mechanisms during cervical cancer progression using fresh tissue samples.
Collected cervical epithelial tissue from 30 patients with LSIL, HSIL, and SCC for transcriptome sequencing.
Identified differentially expressed genes and performed Mfuzz clustering and PPI analysis to find hub genes.
Validated hub gene expression through RT-qPCR in an independent clinical cohort.
Identified 1,654 DEGs (HSIL vs. LSIL) and 6,635 DEGs (SCC vs. HSIL).
Finalized BUB1B, KIF14, and MELK as hub genes validated by GEO analysis.
Gene Set Enrichment Analysis showed significant enrichment of hub genes in various pathways, while no significant immune cell association was observed.

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Multi-stage transcriptome analysis identifies hub genes in cervical cancer, suggesting new biomarker potential.

Li et al. (2026) studied this question.

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