February 4, 1977Science
Liposome-Cell Interaction: Transfer and Intracellular Release of a Trapped Fluorescent Marker
Authors
Discussion
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Implication
In vitro study demonstrates intracellular release and wide distribution of encapsulated 6-carboxyfluorescein in frog retinas and human lymphocytes, indicating effective liposomal delivery.
Key Points
- To evaluate whether small unilamellar lipid vesicles can transfer and release trapped aqueous contents directly into the cytoplasm of living cells.
- Prepared small, unilamellar lipid vesicles containing high, self-quenching concentrations of the fluorescent marker 6-carboxyfluorescein.
- Incubated the prepared vesicles with either isolated frog retinas or human lymphocytes to monitor dye uptake and distribution.
- Fluorescence dispersed broadly throughout the cytoplasm of both frog retinal cells and human lymphocytes following vesicle incubation.
- Intracellular fluorescence confirmed dye dilution, demonstrating that sequestered markers are released from the vesicles upon cellular interaction.
Cite This Study
Weinstein et al. (1977) studied this question.