What question did this study set out to answer?

To sequence the linear plasmid pKO2 from Klebsiella oxytoca and determine whether it functions as a functional temperate bacteriophage.

March 2, 2004Journal of BacteriologyOpen Access

The pKO2 Linear Plasmid Prophage ofKlebsiella oxytoca

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Authors

SCSherwood CasjensUniversity of Utah EGEddie B. GilcreaseUniversity of Utah WHWai Mun HuangUniversity of Utah

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Implication

Genomic analysis reveals that the linear plasmid pKO2 functions as a prophage in Klebsiella oxytoca, indicating conserved replication strategies and SOS response regulation.

Key Points

To sequence the linear plasmid pKO2 from Klebsiella oxytoca and determine whether it functions as a functional temperate bacteriophage.
Sequenced and assembled the complete 51,601-bp nucleotide sequence of the linear plasmid pKO2.
Conducted experimental analyses to verify prophage induction and virion formation (phiKO2).
Annotated 64 predicted genes to assess homology with phage N15 and evaluated LexA binding to host SOS-related gene promoters.
Demonstrated that pKO2 is an active prophage (phiKO2) closely related to phage N15, sharing homologous replication, partitioning, repressor, and protelomerase genes.
Identified distinct structural genes including a phage lambda-like tail featuring an exceptionally large central tip tail fiber protein of 3,433 amino acids.
Showed that phiKO2 carries bacterial dinI and umuD homologues that are divergently transcribed and co-regulated by host LexA binding at a shared promoter site.

Cite This Study

Casjens et al. (2004) studied this question.

synapsesocial.com/papers/6a1054152badbc352affce93 https://doi.org/10.1128/jb.186.6.1818-1832.2004

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Implication

Genomic analysis reveals that the linear plasmid pKO2 functions as a prophage in Klebsiella oxytoca, indicating conserved replication strategies and SOS response regulation.

The pKO2 Linear Plasmid Prophage ofKlebsiella oxytoca

Discussion

Member takes

Implication

Key Points

Cite This Study

The pKO2 Linear Plasmid Prophage ofKlebsiella oxytoca

Discussion

Member takes

Implication

Key Points

Cite This Study

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