What question did this study set out to answer?

Determine the biochemical basis of urinary bladder stone formation in mice carrying the X-linked sparse-fur mutation.

May 1, 1976Proceedings of the National Academy of SciencesOpen Access

Abnormal ornithine carbamoyltransferase in mice having the sparse-fur mutation.

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Authors

RDRobert DeMarsUniversity of Wisconsin–Madison SLS L LeVanUniversity of Wisconsin–Madison BTBeth TrendUniversity of Wisconsin–Madison

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Implication

Laboratory investigation reveals abnormal liver ornithine carbamoyltransferase with altered pH kinetics in sparse-fur mutant mice, indicating an underlying metabolic enzyme defect.

Key Points

Determine the biochemical basis of urinary bladder stone formation in mice carrying the X-linked sparse-fur mutation.
Characterized bladder stone composition in sparse-fur (spf) mice using ultraviolet and infrared spectroscopy, chromatography, melting point analysis, and colorimetric assays.
Assayed hepatic ornithine carbamoyltransferase (EC 2.1.3.3) activity across varying pH levels in mutant and normal mice.
Identified orotic acid as the predominant constituent of urinary bladder stones found in mice carrying the X-chromosomal sparse-fur mutation.
Identified an abnormal form of liver ornithine carbamoyltransferase in spf-bearing mice with altered kinetic characteristics compared to normal enzyme (which exhibits maximal activity at pH 7.6–8.0 and 80% maximal activity at pH 10.0).

Cite This Study

DeMars et al. (1976) studied this question.

synapsesocial.com/papers/6a105f3b5725bbd5cc60ea41 https://doi.org/10.1073/pnas.73.5.1693

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Implication

Laboratory investigation reveals abnormal liver ornithine carbamoyltransferase with altered pH kinetics in sparse-fur mutant mice, indicating an underlying metabolic enzyme defect.

Abnormal ornithine carbamoyltransferase in mice having the sparse-fur mutation.

Discussion

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Implication

Key Points

Cite This Study

Abnormal ornithine carbamoyltransferase in mice having the sparse-fur mutation.

Discussion

Member takes

Implication

Key Points

Cite This Study

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