What question did this study set out to answer?

To establish a rapid, straightforward method using sodium carbonate to isolate intracellular organelle membranes and release luminal contents and peripheral proteins.

April 1, 1982The Journal of Cell BiologyOpen Access

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

View Full Paper

Ask AI

Bookmark

Authors

YFYukio FujikiKyushu University AHA L HubbardUniversity of California, San Francisco SFS FowlerJohns Hopkins University

Discussion

Member takes

Overview

Experimental study demonstrates efficient membrane isolation using sodium carbonate in rat liver microsomes, highlighting effective separation of integral membranes from soluble proteins.

Key Points

To establish a rapid, straightforward method using sodium carbonate to isolate intracellular organelle membranes and release luminal contents and peripheral proteins.
Diluted subcellular organelle fractions in ice-cold 100 mM sodium carbonate (Na2CO3) to convert closed vesicles into flat, open membrane sheets.
Pelleted the resulting membrane sheets through centrifugation while recovering soluble luminal and peripheral proteins in the supernatant.
Applied the procedure to rat liver microsomal subfractions resolved via continuous density gradient centrifugation.
Sodium carbonate treatment converted closed microsomal vesicles into open membrane sheets, releasing internal soluble contents and peripheral membrane proteins without solubilizing the lipid bilayer.
Analysis confirmed and extended the known distribution patterns of specific marker enzymes and structural proteins across smooth and rough endoplasmic reticulum membranes.

Cite This Study

Fujiki et al. (1982) studied this question.

synapsesocial.com/papers/6a1069d2e1a472cb5efcda94 https://doi.org/10.1083/jcb.93.1.97

View Full Paper

Ask AI

Bookmark

April 1, 1982The Journal of Cell BiologyOpen Access

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.

View Full Paper

Ask AI

Bookmark

Authors

YFYukio FujikiKyushu University AHA L HubbardUniversity of California, San Francisco SFS FowlerJohns Hopkins University

Discussion

Member takes

Overview

Experimental study demonstrates efficient membrane isolation using sodium carbonate in rat liver microsomes, highlighting effective separation of integral membranes from soluble proteins.

Key Points

To establish a rapid, straightforward method using sodium carbonate to isolate intracellular organelle membranes and release luminal contents and peripheral proteins.
Diluted subcellular organelle fractions in ice-cold 100 mM sodium carbonate (Na2CO3) to convert closed vesicles into flat, open membrane sheets.
Pelleted the resulting membrane sheets through centrifugation while recovering soluble luminal and peripheral proteins in the supernatant.
Applied the procedure to rat liver microsomal subfractions resolved via continuous density gradient centrifugation.
Sodium carbonate treatment converted closed microsomal vesicles into open membrane sheets, releasing internal soluble contents and peripheral membrane proteins without solubilizing the lipid bilayer.
Analysis confirmed and extended the known distribution patterns of specific marker enzymes and structural proteins across smooth and rough endoplasmic reticulum membranes.

Cite This Study

Fujiki et al. (1982) studied this question.

synapsesocial.com/papers/6a1069d2e1a472cb5efcda94 https://doi.org/10.1083/jcb.93.1.97

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark