Why the study?
Applying multiplex immunofluorescence to human post-mortem tissue requires efficient antibody elution to enable flexible antibody combinations and multiple rounds of immunostaining.
Both 2-Mercaptoethanol/SDS-based and Urea/SDS/Glycine-based elution strategies are effective for multiplex immunofluorescence in post-mortem human tissue.
May support multiplex immunofluorescence in fixed post-mortem tissue; leaves open validation before broader research adoption.
The application of multiplex immunofluorescence to human post-mortem tissue would drive observational studies of selective vulnerability in neurodegenerative proteinopathies. Efficient elution of antibodies is critical for flexibility of antibody combinations and the ability to utilize a sample in multiple rounds of immunostaining. Here, we test two elution strategies for antibodies relevant to the study of selective vulnerability in neurodegenerative diseases in post-mortem human samples from both long-fixed and short-fixed tissue. Both 2-Mercaptoethanol/SDS-based and Urea/SDS/Glycine-based elution strategies work well with the antibodies selected, confirming observations from previous studies with other antibody types.
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Ehrenberg et al. (2019) studied Neurodegenerative diseases. 2-Mercaptoethanol/SDS-based and Urea/SDS/Glycine-based elution strategies was evaluated on Efficacy of elution strategies.
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