What question did this study set out to answer?

To isolate, determine the complete primary amino acid sequence, and evaluate the biological potency of rat hypothalamic corticotropin-releasing factor.

August 1, 1983Proceedings of the National Academy of SciencesOpen Access

Characterization of rat hypothalamic corticotropin-releasing factor.

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Authors

JRJean RivierSalk Institute for Biological Studies JSJoachim SpiessSanford Burnham Prebys Medical Discovery Institute WVW. ValeSalk Institute for Biological Studies

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Implication

Biochemical study reveals the primary sequence and bioactivity of rat hypothalamic corticotropin-releasing factor, demonstrating high structural conservation with ovine CRF.

Key Points

To isolate, determine the complete primary amino acid sequence, and evaluate the biological potency of rat hypothalamic corticotropin-releasing factor.
Purified polypeptide from rat hypothalamic extracts based on ACTH-releasing activity in cultured rat anterior pituitary cells and radioimmunoassay targeting ovine CRF residues 4–20.
Determined primary amino acid sequence using Edman degradation, peptide mapping, and amino acid analysis.
Assessed the comparative hypophysiotropic potency of synthetic rat CRF against isolated native rat CRF and synthetic ovine CRF.
Identified rat CRF as a 41-residue peptide amide: H-Ser-Glu-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Ala-Arg-Ala-Glu-Gln-Leu-Ala-Gln-Gln-Ala-His-Ser-Asn-Arg-Lys-Leu-Met-Glu-Ile-Ile-NH2.
Established an 83% sequence homology between rat and ovine hypothalamic CRF.
Found that the hypophysiotropic potency of synthetic rat CRF did not deviate significantly from the potencies of native rat CRF or synthetic ovine CRF.

Cite This Study

Rivier et al. (1983) studied this question.

synapsesocial.com/papers/6a107e5712e2385ed3fe2a0f https://doi.org/10.1073/pnas.80.15.4851

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Implication

Biochemical study reveals the primary sequence and bioactivity of rat hypothalamic corticotropin-releasing factor, demonstrating high structural conservation with ovine CRF.

Characterization of rat hypothalamic corticotropin-releasing factor.

Discussion

Member takes

Implication

Key Points

Cite This Study

Characterization of rat hypothalamic corticotropin-releasing factor.

Discussion

Member takes

Implication

Key Points

Cite This Study

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