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Dot Blot Enzyme-Linked Immunosorbent Assay for Monitoring the Fate of Insecticidal Toxins from Bacillus thuringiensis in Soil

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Randomized trial monitors the accumulation of insecticidal toxins in soil, suggesting their persistence.

The study aims to monitor the fate of insecticidal toxins from Bacillus thuringiensis in soil environments.
Developed a dot blot enzyme-linked immunosorbent assay (ELISA) to detect free and particle-bound toxins from Bacillus thuringiensis.
Raised antibodies in goats and rabbits specific to toxins from different Bacillus thuringiensis subspecies.
Monitored toxins in sterile and nonsterile soil with various clay minerals over incubation periods.
Detected toxins binding to the clay-particle-size fraction of soil after 40 days in nonsterile conditions.
No toxins were found in silt- and sand-particle-size fractions.
The limit of detection for the toxins was approximately 3 ng, indicating high sensitivity.

Tapp et al. (1995) studied this question.

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