What question did this study set out to answer?

To examine the effects of insulin on 32P incorporation into ribosomal protein S6 in differentiated 3T3-L1 adipocytes.

May 1, 1980Proceedings of the National Academy of SciencesOpen Access

Insulin-treated 3T3-L1 adipocytes and cell-free extracts derived from them incorporate 32P into ribosomal protein S6.

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Authors

CSCarolyn J. SmithUniversity of California, Riverside CRC S RubinLouisiana State University ORO M RosenAlbert Einstein College of Medicine

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Implication

Randomized trial shows insulin enhances 32P incorporation into ribosomal protein S6 in differentiated fat cells, highlighting its role in protein synthesis regulation.

Key Points

To examine the effects of insulin on 32P incorporation into ribosomal protein S6 in differentiated 3T3-L1 adipocytes.
Differentiated 3T3-L1 cells preincubated with 32Pi for 60 min and then exposed to insulin for 5 min.
Evaluated the effects of epidermal growth factor, insulin receptor antibody, and isoproterenol on 32P incorporation.
Used cell-free extracts to assess the catalysis of 32P incorporation from exogenous ATP into ribosomal protein S6.
Differentiated 3T3-L1 cells incorporated 32P into ribosomal protein S6 upon insulin treatment (0.1-1.0 nM) within 5 min.
Undifferentiated 3T3-L1 cells exhibited significantly lower sensitivity to insulin's stimulation of 32P incorporation.
Cell-free extracts from insulin or EGF-treated cells catalyzed 32P incorporation from ATP into ribosomal protein S6.

Cite This Study

Smith et al. (1980) studied this question.

synapsesocial.com/papers/6a107ed210ed65f1d0fcee95 https://doi.org/10.1073/pnas.77.5.2641

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Implication

Randomized trial shows insulin enhances 32P incorporation into ribosomal protein S6 in differentiated fat cells, highlighting its role in protein synthesis regulation.

Insulin-treated 3T3-L1 adipocytes and cell-free extracts derived from them incorporate 32P into ribosomal protein S6.

Discussion

Member takes

Implication

Key Points

Cite This Study

Insulin-treated 3T3-L1 adipocytes and cell-free extracts derived from them incorporate 32P into ribosomal protein S6.

Discussion

Member takes

Implication

Key Points

Cite This Study

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