What does this research mean for the field?

Exogenous myosin can be reconstituted in situ within myosin-extracted skeletal muscle myofibrils to form structures that functionally and structurally resemble native myofibrils and contract in response to ATP. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

February 1, 1982The Journal of Cell BiologyOpen Access

In situ reconstitution of myosin filaments within the myosin-extracted myofibril in cultured skeletal muscle cells.

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Population

Cultured chick embryo skeletal muscle cells (glycerinated, myosin-extracted)

Design

Preclinical

Authors

MTM. TaniguchiNagoya University HIH IshikawaFujita Health University

Discussion

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Implication

Reconstitutes functional sarcomeres in extracted embryonic myofibrils; leaves open whether similar repletion strategies apply to mammalian muscle disease models.

Structured PICO

Population

Cultured chick embryo skeletal muscle cells (glycerinated, myosin-extracted)

Intervention

Addition of rabbit skeletal muscle myosin in high-salt solution (0.6 M KCl), followed by dilution to 0.1 M KCl and addition of Mg-ATP

Outcome

Reconstitution of myosin filaments and restoration of sarcomeric arrangementsurrogate

This study demonstrates that myosin filaments can be reconstituted in situ within myosin-extracted myofibrils, restoring their structural and functional properties.

Cite This Study

Taniguchi et al. (1982) studied this question.

synapsesocial.com/papers/6a109a98d478ddac0ffd420e https://doi.org/10.1083/jcb.92.2.324

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Implication

Reconstitutes functional sarcomeres in extracted embryonic myofibrils; leaves open whether similar repletion strategies apply to mammalian muscle disease models.

In situ reconstitution of myosin filaments within the myosin-extracted myofibril in cultured skeletal muscle cells.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

In situ reconstitution of myosin filaments within the myosin-extracted myofibril in cultured skeletal muscle cells.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider