What question did this study set out to answer?

To investigate whether human preadipocytes express angiotensin II receptors and to determine their functional role in regulating cell cycle progression.

January 1, 1999Endocrinology

Angiotensin II Receptors in Human Preadipocytes: Role in Cell Cycle Regulation

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Authors

DCDavid L. CrandallIdaho National Laboratory DADouglas ArmellinoThe University of Texas MD Anderson Cancer Center DBDennis E. BuslerThomas Jefferson University

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Implication

In vitro study demonstrates that angiotensin II drives cell cycle progression via AT1 receptors in human preadipocytes, suggesting a regulatory role in fat cell differentiation.

Key Points

To investigate whether human preadipocytes express angiotensin II receptors and to determine their functional role in regulating cell cycle progression.
Assessed receptor binding characteristics in primary human preadipocyte cultures using 125I-labeled [Sar1,Ile8]AII.
Evaluated cell cycle progression and cyclin D1 mRNA induction following angiotensin II exposure with and without subtype-selective receptor antagonists.
Confirmed preadipocyte lineage via differentiation medium, tracking leptin mRNA expression and flow cytometric lipid accumulation.
Human preadipocytes exhibited rapid, saturable, and specific high-affinity angiotensin II binding sites characteristic of the AT1 receptor subtype.
Angiotensin II treatment stimulated G1 phase cell cycle progression and induced a dose-dependent increase in cyclin D1 mRNA expression, an effect selectively mediated by the AT1 receptor.

Cite This Study

Crandall et al. (1999) studied this question.

synapsesocial.com/papers/6a10cd6bed67694fb09f7638 https://doi.org/10.1210/endo.140.1.6430

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Implication

In vitro study demonstrates that angiotensin II drives cell cycle progression via AT1 receptors in human preadipocytes, suggesting a regulatory role in fat cell differentiation.

Angiotensin II Receptors in Human Preadipocytes: Role in Cell Cycle Regulation

Discussion

Member takes

Implication

Key Points

Cite This Study

Angiotensin II Receptors in Human Preadipocytes: Role in Cell Cycle Regulation

Discussion

Member takes

Implication

Key Points

Cite This Study

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