What is the clinical evidence from this study?

Study design: Other. Population: Angiotensin II receptor distribution in adipocytes. Intervention: Angiotensin II receptor binding assays. Primary outcome: Angiotensin II receptor binding affinity and number.

What question did this study set out to answer?

To determine the distribution, binding affinity, and pharmacological subtype of angiotensin II receptors across distinct fat depots in rats and humans.

August 1, 1994Journal of Lipid ResearchOpen Access

Distribution of angiotensin II receptors in rat and human adipocytes.

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Key result

Angiotensin II receptors of the AT1 subtype are present in rat and human adipocytes with site-specific differences in receptor number, suggesting a role in peripheral renin-angiotensin metabolism.

Population

Rat adipocytes from epididymal, mesenteric, and retroperitoneal fat depots of male Sprague-Dawley rats at…

Design

Preclinical

Authors

DCDavid L. CrandallIdaho National Laboratory HHH. HerzlingerAmerican Medical Research BSBrian SaundersPennsylvania State University

Discussion

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Implication

AT1 receptors in adipocytes extend RAS localization; leaves open functional and therapeutic implications in humans.

Key Points

To determine the distribution, binding affinity, and pharmacological subtype of angiotensin II receptors across distinct fat depots in rats and humans.
Isolated adipocytes via collagenase digestion and prepared plasma membranes from epididymal, mesenteric, and retroperitoneal depots of 100-day-old male Sprague-Dawley rats, as well as human omental and subcutaneous depots.
Conducted binding assays with 125I-labeled [Sar1,Ile8]AII and pharmacological competition assays using angiotensin peptides, the AT1-selective antagonist losartan, and the AT2-selective antagonist PD 123,319.
Specific and saturable binding was observed in all rat depots with an affinity of approximately 1 nM; receptor density per unit membrane protein was significantly higher in epididymal and mesenteric membranes than retroperitoneal membranes, ranking epididymal > retroperitoneal > mesenteric per cell volume.
Pharmacological profiling identified the AT1 subtype in rat adipocytes, showing significantly greater losartan affinity in mesenteric and retroperitoneal cells compared with epididymal cells.
Human omental and subcutaneous adipocyte membranes both expressed angiotensin II receptors, exhibiting an affinity of approximately 10 nM for losartan.

Structured PICO

Population

Rat adipocytes from epididymal, mesenteric, and retroperitoneal fat depots of male Sprague-Dawley rats at 100 days of age, and human adipocytes from omental and subcutaneous adipose tissue.

Intervention

Angiotensin II receptor binding assays using 125I-labeled [Sar1,Ile8]AII, losartan, and PD 123,319

Outcome

Angiotensin II receptor binding affinity and receptor numbersurrogate

The identification of AT1 receptors in human and rat adipocytes suggests that adipose tissue plays a significant role in the peripheral metabolism of the renin-angiotensin system.

Cite This Study

Crandall et al. (1994) studied Angiotensin II receptor distribution in adipocytes. Angiotensin II receptor binding assays was evaluated on Angiotensin II receptor binding affinity and number. Angiotensin II receptors of the AT1 subtype are present in rat and human adipocytes with site-specific differences in receptor number, suggesting a role in peripheral renin-angiotensin metabolism.

synapsesocial.com/papers/6a15ccd3a2352da34782eca6 https://doi.org/10.1016/s0022-2275(20)40079-3

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Distribution of angiotensin II receptors in rat and human adipocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Distribution of angiotensin II receptors in rat and human adipocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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