In vitro study demonstrates functional expression of the Streptomyces alboniger puromycin resistance gene in mammalian cells, highlighting its utility as a dominant selectable marker.
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.
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Vara et al. (1986) studied this question.
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