Supramaximal Testing to Confirm the Achievement of VO2max in Acute Hypoxia

To confirm maximal oxygen uptake (V̇O 2max ), a bout of supramaximal exercise can be performed after maximal exercise. Supramaximal exercise verifies V̇O 2max if the V̇O 2max is similar to the incremental test. Acute hypoxia decreases V̇O 2max because of decreases in arterial oxygenation, which results in different determinants of V̇O 2max . Purpose We sought to determine if supramaximal exercise testing confirms the achievement of V̇O 2max in acute hypoxia. We hypothesized that the incremental and supramaximal V̇O 2 will be sufficiently similar in acute hypoxia. Methods Twenty-one healthy adults (males n = 13, females n = 8) completed incremental and supramaximal exercise tests in normoxia and acute hypoxia (fraction inspired oxygen = 0.14) separated by at least 48 h. Incremental exercise started at 80 and 60 W in normoxia and 40 and 20 W in hypoxia for males and females, respectively, with all increasing by 20 W each minute until volitional exhaustion. After a 20-min postexercise rest period, a supramaximal test at 110% peak power until volitional exhaustion was completed. Results Supramaximal exercise testing yielded a lower V̇O 2 than incremental testing in hypoxia (3.11 ± 0.78 vs 3.21 ± 0.83 L·min −1 , P = 0.001) and normoxia (3.71 ± 0.91 vs 3.80 ± 1.02 L·min −1 , P = 0.01). Incremental and supramaximal V̇O 2 were statistically similar, using investigator-determined equivalence bounds ±150 mL·min −1 , in hypoxia ( P = 0.02, 90% confidence interval [CI] = 0.05–0.14) and normoxia ( P = 0.03, 90% CI = 0.01–0.14). Likewise, using ±2.1 mL·kg −1 ·min −1 bounds, incremental and supramaximal V̇O 2 values were statistically similar in hypoxia ( P = 0.04, 90% CI = 0.70–2.0) and normoxia ( P = 0.04, 90% CI = 0.30–2.0). Conclusions Despite differences in the oxygen cascade, incremental and supramaximal V̇O 2 values were statistically similar in both hypoxia and normoxia, demonstrating the utility of supramaximal verification of V̇O 2max in the setting of acute hypoxia.